Aged garlic extract modulates glutathione redox cycle and superoxide dismutase activity in vascular endothelial cells

1997 ◽  
Vol 11 (1) ◽  
pp. 54-56 ◽  
Author(s):  
Zhaohui Geng ◽  
Benjamin H. S. Lau
Keyword(s):  
Endothelial Cells ◽  
Superoxide Dismutase ◽  
Vascular Endothelial Cells ◽  
Garlic Extract ◽  
Vascular Endothelial ◽  
Redox Cycle ◽  
Aged Garlic Extract ◽  
Glutathione Redox Cycle ◽  
Glutathione Redox ◽  
Aged Garlic

Peroxynitrite modulates receptor-activated Ca2+ signaling in vascular endothelial cells

1996 ◽  
Vol 270 (6) ◽  
pp. L954-L961 ◽  
Author(s):  
S. J. Elliott
Keyword(s):  
Endothelial Cells ◽  
Superoxide Dismutase ◽  
Endothelial Cell ◽  
Vascular Endothelial Cells ◽  
Inhibitory Effect ◽  
Vascular Endothelial ◽  
No Donor ◽  
Time Points ◽  
Inactive Metabolite ◽  
Spermine Nonoate

Peroxynitrite (ONOO-) is formed from superoxide (O2-) and .NO. We have previously reported that O2- does not alter endothelial cell Ca2+ signaling. To test whether .NO alters Ca2+ signaling, cells were incubated with the .NO donor, spermine NONOate. Neither spermine NONOate nor S-nitroso-N-acetyl penicillamine (SNAP) altered bradykinin-stimulated Ca2+ signaling. By contrast, 3-morpholinosydnonimine (SIN-1), which generates ONOO- by releasing O2- and .NO essentially in a simultaneous manner, significantly inhibited signaling. Initially, the inhibitory effect of 1 mM SIN-1 was selective toward agonist-stimulated influx of external Ca2+. At later time points, SIN-1 additionally depleted internal stores of releasable Ca2+. When cells were coincubated with SIN-1 plus superoxide dismutase, a technique designed to scavenge O2- and convert SIN-1 to purely an .NO-donor compound, Ca2+ signaling was identical to control. SIN-1C, the inactive metabolite of SIN-1, had no effect on [Ca2+]i. This study demonstrates that exogenously generated ONOO- modulates endothelial cell Ca2+ signaling, suggesting that ONOO- is of biological relevance to vasoregulation.

Effect of medium pH on glutathione redox cycle in cultured human umbilical vein endothelial cells

Metabolism ◽  
1993 ◽  
Vol 42 (9) ◽  
pp. 1121-1126 ◽  
Author(s):  
Motoyoshi Ikebuchi ◽  
Atsunori Kashiwagi ◽  
Takayuki Asahina ◽  
Yasushi Tanaka ◽  
Yoshihumi Takagi ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Umbilical Vein ◽  
Redox Cycle ◽  
Medium Ph ◽  
Human Umbilical Vein ◽  
Glutathione Redox Cycle ◽  
Umbilical Vein Endothelial Cells ◽  
Glutathione Redox
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